Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 17-9015-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively.
- Antibody clone number
- M4G3LN
- Concentration
- 5 µL/Test
Submitted references Complement Factor H Modulates Splenic B Cell Development and Limits Autoantibody Production.
Kiss MG, Ozsvár-Kozma M, Porsch F, Göderle L, Papac-Miličević N, Bartolini-Gritti B, Tsiantoulas D, Pickering MC, Binder CJ
Frontiers in immunology 2019;10:1607
Frontiers in immunology 2019;10:1607
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Mouse splenocytes were unstimulated (left), 5-minute F (ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified-stimulated (Product # 16-5092-85) (middle), or hydrogen peroxide-activated sodium pervanadate-treated (right) then intracellularly stained with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and Anti-Human/Mouse phospho-BTK/ITK (Y551/Y511) APC using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol.