Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-54912 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenosine Deaminase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCC
- Concentration
- 0.1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Adenosine Deaminase in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Adenosine Deaminase in human cell line U-2 OS shows positivity in plasma membrane & cytoplasm. Samples were probed using an Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human lymph node using Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human duodenum and liver tissues using Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912). Corresponding ADA RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human colon, duodenum, lymph node and testis using Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912) (A) shows similar protein distribution across tissues to an independent Adenosine Deaminase Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human colon using Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912) shows no positivity in glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human testis using Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912) shows no positivity in cells in seminiferous ducts as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human duodenum using Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912) shows moderate to strong cytoplasmic and nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Adenosine Deaminase in human liver using Adenosine Deaminase Polyclonal Antibody (Product # PA5-54912) shows no positivity in hepatocytes as expected.
