Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31262 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRE11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, A431, C8D30, NIH-3T3, PC-12.
- Concentration
- 0.57 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MRE11 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MRE11 Polyclonal Antibody (Product # PA5-31262) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MRE11 Polyclonal Antibody (Product # PA5-31262). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. MRE11 Polyclonal Antibody (Product # PA5-31262) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MRE11 Polyclonal Antibody detects MRE11A protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract. B. 30 µg NIH-3T3 whole cell lysate/extract.7.5 % SDS-PAGE. MRE11 Polyclonal Antibody (Product # PA5-31262) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MRE11 Polyclonal Antibody detects MRE11A protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.7.5 % SDS-PAGE. MRE11 Polyclonal Antibody (Product # PA5-31262) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Mre11 in paraformaldehyde-fixed A431 cells using a Mre11 polyclonal antibody (Product # PA5-31262) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Mre11 (Product # PA5-31262) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.