MA1-23253

antibody from Invitrogen Antibodies

Targeting: MRE11 ATLD, MRE11A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Recommended by provider)

Antibody Data

Product number

MA1-23253

Provider

Invitrogen Antibodies

Product name

MRE11 Monoclonal Antibody (12D7)

Antibody type

Monoclonal

Antigen

Other

Description

Recommended positive controls: HeLa, Jurkat, Raji, NCI-H929, 293T, human Mre11-transfected 293T, PC-12.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

12D7

Vial size

100 µL

Concentration

0.45 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRE11 using A) 30 µg Jurkat whole cell extract (B) 30 µg Raji whole cell extract and C) 30 µg NCI-H929 whole cell extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MRE11 monoclonal antibody (Product # MA1-23253) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRE11 using 30 µg HeLa whole cell extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MRE11 monoclonal antibody (Product # MA1-23253) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRE11 in HeLa (1) and Raji (2) whole cell extracts. Samples were probed with a MRE11 monoclonal antibody (Product # MA1-23253).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MRE11 Monoclonal Antibody (12D7) detects Mre11 protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg whole cell extract of human Mre11-transfected 293T cells.7.5% SDS-PAGE. MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253) dilution: 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MRE11 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253) at a dilution of 1:1000 and a HRP-conjugated anti-mouse IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MRE11 Monoclonal Antibody (12D7) detects Mre11 protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg whole cell extract of human Mre11-transfected 293T cells.7.5% SDS-PAGE. MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253) dilution: 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MRE11 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MRE11 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MRE11 Monoclonal Antibody (12D7) detects Mre11 protein at nucleus by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human colon cancer. Mre11 stained by MRE11 Monoclonal Antibody (12D7) (Product # MA1-23253) diluted at 1:300. Antigen Retrieval: EDTA buffer, 20 min.