LS-C355704

antibody from LSBio

Targeting: SNAP25 bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C355704

Provider

LSBio

Product name

SNAP25 Antibody LS-C355704

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Mouse, Rat, Bovine, Chicken/Avian, Guinea Pig, Hamster, Zebrafish

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2), Rat Brain (Lane 3) and membrane enriched extract (30 µg lysate) of PC-12 (Lane 4). The blot was probed with Anti-SNAP25 Rabbit Polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A ~25 kDa band corresponding to SNAP25 was observed across the tissues and cell line tested. Known quantity of protein samples were electrophoresed using 12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk.