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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA3-104 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-NMDAR2B (Tyr1122) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA3-104 detects N-methyl-D-aspartate (NMDA) receptor type 2B.
- Concentration
- Conc. Not Determined
Submitted references Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.
Impacts of forebrain neuronal glycine transporter 1 disruption in the senescent brain: evidence for age-dependent phenotypes in Pavlovian learning.
Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, Boczek T
Frontiers in cellular neuroscience 2017;11:181
Frontiers in cellular neuroscience 2017;11:181
Impacts of forebrain neuronal glycine transporter 1 disruption in the senescent brain: evidence for age-dependent phenotypes in Pavlovian learning.
Dubroqua S, Singer P, Boison D, Feldon J, Möhler H, Yee BK
Behavioral neuroscience 2010 Dec;124(6):839-50
Behavioral neuroscience 2010 Dec;124(6):839-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence image of NMDA receptor type 2B in rat brain tissue using Product # PA3-104.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence image of NMDA receptor type 2B in rat brain tissue using a Phospho-NMDAR2B (Tyr1122) polyclonal antibody (Product # PA3-104).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Interaction of PSD95 with NMDAR subunits and PMCA isoforms. Brain lysates were incubated with anti-PSD95 antibodies conjugated to sepharose beads. Immunocomplexes were recovered and resolved by SDS-PAGE and the membranes were next probed with anti-NMDAR1 (A) , anti-NMDAR2A (B) , anti-NMDAR2B (C) , 5F10 (D) , anti-PMCA2 (E) or anti-PMCA4 (F) antibodies. Negative controls included sepharose-linked secondary antibodies (Control Ab) or sepharose beads only. PSD95 was also immunoprobed in beads-unbound fraction (unbound). Representative blots are shown. The bands intensity were densitometically quantified and the results were expressed as AU defined as the optical density per mg of protein (OD/mg protein). * P < 0.05 ketamine treated vs. saline, n = 5. CX, cortex; CB, cerebellum; H, hippocampus; ST, striatum.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Western blot analysis of PSD95 and N-methyl-D-aspartic acid receptor (NMDAR) subunit protein level in synaptosomal membranes. The protein level of PSD95, NMDAR1, NMDAR2A and NMDAR2B was determined by immunoblotting (A) and the bands intensity corresponding to the target protein was densitometrically analyzed (B) . The results are expressed as AU obtained following normalization to endogenous Na + /K + -ATPase level. Representative blots are shown. * P < 0.05 ketamine treated vs. saline, n = 5. CX, cortex; CB, cerebellum; H, hippocampus; ST, striatum.
