PAB29309

antibody from Abnova Corporation

Targeting: PIK3R1 GRB1, p85, p85-ALPHA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29309

Provider

Abnova Corporation

Product name

PIK3R1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human PIK3R1.

Antigen sequence

LADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSS
EEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLS
QTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENL
IKVIEILISTEWNERQPAPALPPKPPKPTTVANNG
MNNNMSL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with PIK3R1 polyclonal antibody (Cat# PAB29309) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence staining of U-251 MG cells with PIK3R1 polyclonal antibody (Cat# PAB29309) under 1-4 ug/mL working concentration.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach tissue with PIK3R1 polyclonal antibody (Cat# PAB29309) at 1:50 - 1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)