NBP2-52685-0.2mg

antibody from Novus Biologicals

Targeting: EGFR ERBB, ERBB1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NBP2-52685-0.2mg

Provider

Novus Biologicals

Product name

Rabbit Monoclonal EGFR Antibody

Antibody type

Monoclonal

Description

Protein A purified. Binds to human EGFR.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.2 mg

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: EGFR Antibody (Matuzumab) [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.