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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- BAF231 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EGFR Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EGFR in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse EGFR and less than 0.1% cross-reactivity with recombinant human (rh) EGF, rhErbB2, and rhErbB3.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
CAA25240- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers.
Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1.
Protein shedding in urothelial bladder cancer: prognostic implications of soluble urinary EGFR and EpCAM.
SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Lee H, Choi M, Lim J, Jo M, Han JY, Kim TM, Cho Y
Theranostics 2018;8(2):505-517
Theranostics 2018;8(2):505-517
Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1.
Sakanyan V, Hulin P, Alves de Sousa R, Silva VA, Hambardzumyan A, Nedellec S, Tomasoni C, Logé C, Pineau C, Roussakis C, Fleury F, Artaud I
Scientific reports 2016 Feb 17;6:21088
Scientific reports 2016 Feb 17;6:21088
Protein shedding in urothelial bladder cancer: prognostic implications of soluble urinary EGFR and EpCAM.
Bryan RT, Regan HL, Pirrie SJ, Devall AJ, Cheng KK, Zeegers MP, James ND, Knowles MA, Ward DG
British journal of cancer 2015 Mar 17;112(6):1052-8
British journal of cancer 2015 Mar 17;112(6):1052-8
SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR.
Thompson CM, Bloom LR, Ogiue-Ikeda M, Machida K
BMC biotechnology 2015 Jun 26;15:60
BMC biotechnology 2015 Jun 26;15:60
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of EGFR in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human EGFR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF231, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).
