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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX32252 - Provider product page
- Provider
- GeneTex
- Product name
- FAK (phospho Tyr925) antibody
- Antibody type
- Polyclonal
- Reactivity
- Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of Focal Adhesion Kinase (pY925) expression in NIH3T3 pervanadate-treated (A) whole cell lysates.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunofluorescent analysis of Focal Adhesion Kinase (pY925) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
