H00005747-D01P

antibody from Abnova Corporation

Targeting: PTK2 FADK, FAK, FAK1, PPP1R71

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005747-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005747-D01P, RRID:AB_1579334

Product name

PTK2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PTK2 protein.

Antigen sequence

MKYQEVRCLTSFNISVSFPAETDDYAEIIDEEDTY
TMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYM
SPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQ
FDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQ
VRKYSLDLASLILYAYQLSTALAYLESKRFVHRDI
AARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKG
KLPIKWMAPESINFRRFTSASDVWMFGVCMWEILM
HGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLY
SLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQ
EERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPS
PRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMA
GSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDST
VLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLE
KEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQ
PVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADS
YNEGVKLKPQEISPPPTANLDRSNDKVYENVTGLV
KAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLAT
VDETIPLLPASTHREIEMAQKLLNSDLGELINKMK
LAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDV
IDQARLKMLGQTRRH


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PTK2 expression in transfected 293T cell line (H00005747-T01) by PTK2 MaxPab polyclonal antibody.Lane 1: PTK2 transfected lysate(76.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PTK2 and FLT1. Huh7 cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-FLT1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PTK2 and PLCG1. Mahlavu cells were stained with anti-PTK2 rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PTK2 and APC. HeLa cells were stained with anti-PTK2 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)