PA5-35321
antibody from Invitrogen Antibodies
Targeting: GTF2I
BAP-135, BTKAP1, DIWS, IB291, SPIN, TFII-I, WBSCR6
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-35321 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTF2I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GTF2I in Hela cell lysate (35 µg/lane) using a GTF2I polyclonal antibody (Product # PA5-35321).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GTF2I showing staining in the nucleus of A549 cells using a GTF2I polyclonal antibody (Product # PA5-35321) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37ºC).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of GTF2I in formalin-fixed, paraffin-embedded human brain tissue using a GTF2I polyclonal antibody (Product # PA5-35321) followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of GTF2I in K562 cells (bottom) compared to a negative control (top) using a GTF2I polyclonal antibody (Product # PA5-35321) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.