Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA1-41141 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMAD1/SMAD5/SMAD9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, chimpanzee, invertabrates, mammals, porcine, xenopus and zebrafish samples based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMADs in lysate from A549 cells probed with a SMAD1, 5, 8, 9 polyclonal antibody (Product # PA1-41141) at 0.5 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMAD1/SMAD5/SMAD9 in lysate from A549 cell. Samples were incubated in SMAD1/SMAD5/SMAD9 polyclonal antibody (Product # PA1-41141) using a dilution of 0.5 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.