Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-22358 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntaxin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, K562, THP-1, HL-60.
- Concentration
- 0.7 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Syntaxin 4 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Syntaxin 4 Polyclonal Antibody (Product # PA5-22358) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Syntaxin 4 Polyclonal Antibody (Product # PA5-22358). Sample (30 µg of whole cell lysate). Lane A: A431. 10% SDS PAGE. Syntaxin 4 Polyclonal Antibody (Product # PA5-22358) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Syntaxin 4 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: Syntaxin 4 Polyclonal Antibody (Product # PA5 22358) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker. Blue: Fluoroshield with DAPI.