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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- MA5-24657 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLE3 Monoclonal Antibody (CL3573)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GSQAHLTVKD EKNHHELDHR ERESSANNSV SPSESLRASE KHR
- Antibody clone number
- CL3573
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of TLE Pan in Lane1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Human cell line HEK 293 Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLE Pan in Lane1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Human cell line HeLa nuclear extract. Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using TLE3 Monoclonal Antibody (CL3573) (Product # MA5-24657). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TLE3 in HeLa cells using TLE3 Monoclonal Antibody (CL3573) (Product # MA5-24657), showing specific staining in the nucleoplasm and nucleoi in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TLE Pan in human cervix shows strong nuclear immunoreactivity in epithelial cells. Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TLE Pan in human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells. Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TLE Pan in human oral mucosa shows strong nuclear positivity in epithelial cells. Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TLE Pan in human small intestine shows moderate nuclear immunoreactivity in glandular epithelium cells. Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TLE Pan in human kidney tissue shows weak to moderate nuclear positivity in a subset of cells. Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TLE Pan in human skeletal muscle shows absence of immunoreactivity (negative control). Samples were probed using a TLE Pan Monoclonal Antibody (Product # MA5-24657).
