Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-9014-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Syk (Tyr348) Monoclonal Antibody (moch1ct), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This moch1ct monoclonal antibody recognizes human and mouse spleen tyrosine kinase (also known as SYK) when phosphorylated on tyrosine 348 (Y348). SYK is the founding member of the SYK family of kinases that also includes ZAP-70 (zeta-associated protein of 70 kD) and is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. SYK is critical for B cell receptor (BCR) signaling and B cell development. Autophosphorylation of at Y348 is necessary for SYK to become fully catalytically active and creates a docking site for the SH2 domains of Vav, Grb2, p85 subunit of PI3 kinase, and PLC gamma.
- Conjugate
- Yellow dye
- Antibody clone number
- moch1ct
- Concentration
- 5 µL/Test
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TOP: Intracellular staining of unstimulated (left) or 2-minute F (ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified-stimulated (Product # 16-5092-85) (right) mouse spleen cells with Anti-Mouse CD45R (B220) APC (Product # 17-0452-82) and Anti-Human/Mouse phospho-SYK (Y348) PE using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. BOTTOM: Intracellular staining of unstimulated (orange histogram), 2-minute F (ab')2 Anti-Mouse IgM, u chain specific Functional Grade Purified-stimulated (Product # 16-5092-85) (purple histogram), or hydrogen peroxide-activated sodium pervanadate-treated mouse spleen cells (green histogram) with Anti-Human/Mouse phospho-SYK (Y348) PE. CD45R (B220)+ lymphocytes were used for analysis.
- Conjugate
- Yellow dye