14-6696-82

antibody from Invitrogen Antibodies

Targeting: SYK

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-6696-82

Provider

Invitrogen Antibodies

Product name

Syk Monoclonal Antibody (4D10.1), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers.

Antibody clone number

4D10.1

Concentration

0.5 mg/mL

References

Submitted references

Specific human cytomegalovirus signature detected in NK cell metabolic changes post vaccination.

Woods E, Zaiatz-Bittencourt V, Bannan C, Bergin C, Finlay DK, Hoffmann M, Brown A, Turner B, Makvandi-Nejad S, Vassilev V, Capone S, Folgori A, Hanke T, Barnes E, Dorrell L, Gardiner CM, PEACHI Consortium
NPJ vaccines 2021 Sep 28;6(1):117

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GSK3 Inhibition Drives Maturation of NK Cells and Enhances Their Antitumor Activity.

Cichocki F, Valamehr B, Bjordahl R, Zhang B, Rezner B, Rogers P, Gaidarova S, Moreno S, Tuininga K, Dougherty P, McCullar V, Howard P, Sarhan D, Taras E, Schlums H, Abbot S, Shoemaker D, Bryceson YT, Blazar BR, Wolchko S, Cooley S, Miller JS
Cancer research 2017 Oct 15;77(20):5664-5675

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Syk and pTyr'd: Signaling through the B cell antigen receptor.

Geahlen RL
Biochimica et biophysica acta 2009 Jul;1793(7):1115-27

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Syk tyrosine kinase is linked to cell motility and progression in squamous cell carcinomas of the head and neck.

Luangdilok S, Box C, Patterson L, Court W, Harrington K, Pitkin L, Rhŷs-Evans P, O-charoenrat P, Eccles S
Cancer research 2007 Aug 15;67(16):7907-16

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Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression.

Chu DH, van Oers NS, Malissen M, Harris J, Elder M, Weiss A
Journal of immunology (Baltimore, Md. : 1950) 1999 Sep 1;163(5):2610-20

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The Syk protein tyrosine kinase can function independently of CD45 or Lck in T cell antigen receptor signaling.

Chu DH, Spits H, Peyron JF, Rowley RB, Bolen JB, Weiss A
The EMBO journal 1996 Nov 15;15(22):6251-61

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoblotting of reduced Ramos cell lysate with 5 µg/mL of Anti-Human Syk antibody. Bands were visualized using Anti-Mouse IgG HRP.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 HCMV impacts on functional and metabolic responses of CD56 dim NK cell subsets post vaccination. Summary of canonical and adaptive CD56 dim NK cells responses at baseline and post-priming vaccination (Day 0, Day 7 and Day 28). Canonical (FcepsilonRgamma1+Syk+) or adaptive (FcepsilonRgamma1+Syk-, FcepsilonRgamma1-Syk+ and FcepsilonRgamma1-Syk-) cells were analysed as indicated for a ex vivo frequencies of NK subsets, b frequency of pS6+, c IFNgamma+ and d IFNgamma MFI in response to IL-12/IL-15 stimulation after vaccination ( n = 4). e Summary of post-priming Mitotracker and f ATP5B MFI in canonical and adaptive CD56 dim NK cells at baseline and Days 7 and 28 ( n = 4). Error bars show SEM. a , e , f Samples were compared by mixed-model with Bonferroni post hoc test. b - d Samples were compared by two-way ANOVA with Sidek's post hoc test. n.s. not significant.