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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- LS-C369913 - Provider product page
- Provider
- LSBio
- Product name
- p56lck / LCK Antibody (aa1-539) LS-C369913
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Immunoprecipitating LCK in Jurkat whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of LCK Antibody in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: LCK Antibody (8µg) + Jurkat whole cell lysate (500µg) Lane 3: Jurkat whole cell lysate (10µg)
