Antibody data
- Antibody Data
- Antigen structure
- References [23]
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- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 11-0457-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45RO Monoclonal Antibody (UCHL1), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.
- Conjugate
- Green dye
- Antibody clone number
- UCHL1
- Concentration
- 5 µL/Test
Submitted references Longitudinal analysis of subtype C envelope tropism for memory CD4(+) T cell subsets over the first 3 years of untreated HIV-1 infection.
Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.
FUNDAMANT: an interventional 72-week phase 1 follow-up study of AADvac1, an active immunotherapy against tau protein pathology in Alzheimer's disease.
Th2/1 Hybrid Cells Occurring in Murine and Human Strongyloidiasis Share Effector Functions of Th1 Cells.
A different representation of natural T cells and natural killer cells between tumor-infiltrating and periphery lymphocytes in human hepatocellular carcinoma.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.
Durable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy.
Impaired survival of regulatory T cells in pulmonary sarcoidosis.
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.
Do patients with a failed metal-on-metal hip implant with a pseudotumor present differences in their peripheral blood lymphocyte subpopulations?
Functional expression of CD137 (4-1BB) on T helper follicular cells.
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
Increased Th17 cells in flow cytometer-sorted CD45RO-positive memory CD4 T cells from patients with systemic lupus erythematosus.
Expression of the memory marker CD45RO on helper T cells in macaques.
The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.
Central role of JC virus-specific CD4+ lymphocytes in progressive multi-focal leucoencephalopathy-immune reconstitution inflammatory syndrome.
Differential expression of CD300a/c on human TH1 and TH17 cells.
Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.
Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.
Frequency of gC1qR+CD4+ T cells increases during acute hepatitis C virus infection and remains elevated in patients with chronic infection.
Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
The effects of trastuzumab on the CD4+CD25+FoxP3+ and CD4+IL17A+ T-cell axis in patients with breast cancer.
Gartner MJ, Gorry PR, Tumpach C, Zhou J, Dantanarayana A, Chang JJ, Angelovich TA, Ellenberg P, Laumaea AE, Nonyane M, Moore PL, Lewin SR, Churchill MJ, Flynn JK, Roche M
Retrovirology 2020 Aug 6;17(1):24
Retrovirology 2020 Aug 6;17(1):24
Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.
Lowe MM, Boothby I, Clancy S, Ahn RS, Liao W, Nguyen DN, Schumann K, Marson A, Mahuron KM, Kingsbury GA, Liu Z, Munoz Sandoval P, Rodriguez RS, Pauli ML, Taravati K, Arron ST, Neuhaus IM, Harris HW, Kim EA, Shin US, Krummel MF, Daud A, Scharschmidt TC, Rosenblum MD
JCI insight 2019 Dec 19;4(24)
JCI insight 2019 Dec 19;4(24)
FUNDAMANT: an interventional 72-week phase 1 follow-up study of AADvac1, an active immunotherapy against tau protein pathology in Alzheimer's disease.
Novak P, Schmidt R, Kontsekova E, Kovacech B, Smolek T, Katina S, Fialova L, Prcina M, Parrak V, Dal-Bianco P, Brunner M, Staffen W, Rainer M, Ondrus M, Ropele S, Smisek M, Sivak R, Zilka N, Winblad B, Novak M
Alzheimer's research & therapy 2018 Oct 24;10(1):108
Alzheimer's research & therapy 2018 Oct 24;10(1):108
Th2/1 Hybrid Cells Occurring in Murine and Human Strongyloidiasis Share Effector Functions of Th1 Cells.
Bock CN, Babu S, Breloer M, Rajamanickam A, Boothra Y, Brunn ML, Kühl AA, Merle R, Löhning M, Hartmann S, Rausch S
Frontiers in cellular and infection microbiology 2017;7:261
Frontiers in cellular and infection microbiology 2017;7:261
A different representation of natural T cells and natural killer cells between tumor-infiltrating and periphery lymphocytes in human hepatocellular carcinoma.
Li XF, Dai D, Song XY, Liu JJ, Zhu L, Zhu X, Ma W, Xu W
Oncology letters 2017 May;13(5):3291-3298
Oncology letters 2017 May;13(5):3291-3298
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.
Tomić A, Varanasi PR, Golemac M, Malić S, Riese P, Borst EM, Mischak-Weissinger E, Guzmán CA, Krmpotić A, Jonjić S, Messerle M
PLoS pathogens 2016 Dec;12(12):e1006015
PLoS pathogens 2016 Dec;12(12):e1006015
Durable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy.
Wyluda EJ, Cheng J, Schell TD, Haley JS, Mallon C, Neves RI, Robertson G, Sivik J, Mackley H, Talamo G, Drabick JJ
Cancer biology & therapy 2015;16(5):662-70
Cancer biology & therapy 2015;16(5):662-70
Impaired survival of regulatory T cells in pulmonary sarcoidosis.
Broos CE, van Nimwegen M, Kleinjan A, ten Berge B, Muskens F, in 't Veen JC, Annema JT, Lambrecht BN, Hoogsteden HC, Hendriks RW, Kool M, van den Blink B
Respiratory research 2015 Sep 16;16(1):108
Respiratory research 2015 Sep 16;16(1):108
Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.
Katawa G, Layland LE, Debrah AY, von Horn C, Batsa L, Kwarteng A, Arriens S, W Taylor D, Specht S, Hoerauf A, Adjobimey T
PLoS neglected tropical diseases 2015 Jan;9(1):e3414
PLoS neglected tropical diseases 2015 Jan;9(1):e3414
Do patients with a failed metal-on-metal hip implant with a pseudotumor present differences in their peripheral blood lymphocyte subpopulations?
Catelas I, Lehoux EA, Hurda I, Baskey SJ, Gala L, Foster R, Kim PR, Beaulé PE
Clinical orthopaedics and related research 2015 Dec;473(12):3903-14
Clinical orthopaedics and related research 2015 Dec;473(12):3903-14
Functional expression of CD137 (4-1BB) on T helper follicular cells.
Alfaro C, Echeveste JI, Rodriguez-Ruiz ME, Solorzano JL, Perez-Gracia JL, Idoate MA, Lopez-Picazo JM, Sanchez-Paulete AR, Labiano S, Rouzaut A, Oñate C, Aznar A, Lozano MD, Melero I
Oncoimmunology 2015 Dec;4(12):e1054597
Oncoimmunology 2015 Dec;4(12):e1054597
Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.
Ivanova I, Seledtsova G, Mamaev S, Shishkov A, Seledtsov V
Human vaccines & immunotherapeutics 2014;10(5):1221-7
Human vaccines & immunotherapeutics 2014;10(5):1221-7
Increased Th17 cells in flow cytometer-sorted CD45RO-positive memory CD4 T cells from patients with systemic lupus erythematosus.
Liu MF, Wang CR
Lupus science & medicine 2014;1(1):e000062
Lupus science & medicine 2014;1(1):e000062
Expression of the memory marker CD45RO on helper T cells in macaques.
Valentine M, Song K, Maresh GA, Mack H, Huaman MC, Polacino P, Ho O, Cristillo A, Kyung Chung H, Hu SL, Pincus SH
PloS one 2013;8(9):e73969
PloS one 2013;8(9):e73969
The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.
Qi Y, Operario DJ, Georas SN, Mosmann TR
PloS one 2012;7(6):e39072
PloS one 2012;7(6):e39072
Central role of JC virus-specific CD4+ lymphocytes in progressive multi-focal leucoencephalopathy-immune reconstitution inflammatory syndrome.
Aly L, Yousef S, Schippling S, Jelcic I, Breiden P, Matschke J, Schulz R, Bofill-Mas S, Jones L, Demina V, Linnebank M, Ogg G, Girones R, Weber T, Sospedra M, Martin R
Brain : a journal of neurology 2011 Sep;134(Pt 9):2687-702
Brain : a journal of neurology 2011 Sep;134(Pt 9):2687-702
Differential expression of CD300a/c on human TH1 and TH17 cells.
Simhadri VR, Mariano JL, Zhou Q, DeBell KE, Borrego F
BMC immunology 2011 Nov 2;12:62
BMC immunology 2011 Nov 2;12:62
Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.
Bentebibel SE, Schmitt N, Banchereau J, Ueno H
Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 16;108(33):E488-97
Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 16;108(33):E488-97
Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.
Narayanan S, Silva R, Peruzzi G, Alvarez Y, Simhadri VR, Debell K, Coligan JE, Borrego F
PloS one 2010 May 13;5(5):e10636
PloS one 2010 May 13;5(5):e10636
Frequency of gC1qR+CD4+ T cells increases during acute hepatitis C virus infection and remains elevated in patients with chronic infection.
Cummings KL, Rosen HR, Hahn YS
Clinical immunology (Orlando, Fla.) 2009 Sep;132(3):401-11
Clinical immunology (Orlando, Fla.) 2009 Sep;132(3):401-11
Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.
Brucklacher-Waldert V, Steinbach K, Lioznov M, Kolster M, Hölscher C, Tolosa E
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5494-501
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5494-501
The effects of trastuzumab on the CD4+CD25+FoxP3+ and CD4+IL17A+ T-cell axis in patients with breast cancer.
Horlock C, Stott B, Dyson PJ, Morishita M, Coombes RC, Savage P, Stebbing J
British journal of cancer 2009 Apr 7;100(7):1061-7
British journal of cancer 2009 Apr 7;100(7):1061-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Anti-Human CD45RO FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Transitional memory and effector memory cells were most frequently infected by C-HIV Envs. a Each data point represents the percentage of infected CD4 + T cells with one pseudovirus (averaged from four independent seronegative blood bank donors). The Env donor is indicated as follows; CAP88 (blue circles), CAP177 (red squares), CAP228 (green triangles), CAP255 (purple inverted triangles) and CAP257 (orange diamonds). Black lines represents the median of all pseudoviruses within each time point. Comparisons were made using a Kruskal-Wallis test with Dunn's post hoc test for multiple comparisons. b Stacked bar graphs represent the contribution of each T cell subset to the pool of infected CD4 + T cells. Values represent the median percentage of infected CD4 + T-cells (averaged across four HIV-seronegative PBMC donors) that belong to the indicated subset [naive; dark blue, T stem cell memory (TSCM); red, central memory (CM); yellow, transitional memory (TM); light blue, effector memory (EM); purple and terminally differentiated (TD); green], and are stratified by participant and time point. Error bars represent the interquartile range. c Dot plot representing the proportion of each T cell subset contributing to the total pool of infected cells for all Env-pseudoviruses. Each point represents a single virus averaged across four seronegative donors, lines represent median and error bars represent interquartile range. Comparisons were made using a Kruskal-Wallis test with Du
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 PITPNM3 is a CCL18 receptor on naive CD4 + T cells. (A) Representative flow cytometry staining for PITPNM3 and CCR8, potential CCL18 receptors, on gated PB T cell subsets and paired TI naive CD4 + T cells of a breast cancer patient. Cells were gated on CD3 + CD45RA + CD45RO - CD25 - CD4 + /CD8 + for naive CD4 + /CD8 + T cells, CD3 + CD45RA - CD45RO + CD25 - CD4 + /CD8 + for memory CD4 + /CD8 + T cells and CD3 + CD4 + CD25 + for Tregs). Quantitation of PITPNM3 and CCR8 expression on T cell subsets for eight breast cancer patients is provided in Supplementary information, Figure S8A . (B-F) Knockdown of PITPNM3 in naive CD4 + T cells inhibits CCL18 binding, signaling and chemotaxis. (B) Binding of 125 I-CCL18 to naive CD4 + T cells, knocked down or not for PITPNM3 (shPI-1,2) in the presence of increasing concentrations of unlabeled CCL18. Shown are the representative assays for three independent experiments using PB T cells from three normal donors. (C) Representative fluorescence microscopy images of CCL18 binding to naive CD4 + T cells, knocked down or not for PITPNM3 , stained for PITPNM3 and CCL18 3 h after adding CCL18. Scale bar, 5 mum. Shown are the representative images for three independent experiments using PB T cells from three normal donors. (D) Immunoblot of CCL18-treated naive CD4 + T cells, knocked down or not for PITPNM3 , showing expression of PITPNM3 and phosphorylated/total (t-) Erk1/2 and Akt, relative to GAPDH as a loading control. Blots are repres
- Conjugate
- Green dye