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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [1]
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- Product number
- 12-0457-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD45RO Monoclonal Antibody (UCHL1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells. Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- UCHL1
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Clinically compliant spatial and temporal imaging of chimeric antigen receptor T-cells.
Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer.
Somatic Variation of T-Cell Receptor Genes Strongly Associate with HLA Class Restriction.
Functional expression of CD137 (4-1BB) on T helper follicular cells.
Delta-like 1-mediated Notch signaling enhances the in vitro conversion of human memory CD4 T cells into FOXP3-expressing regulatory T cells.
Expression of the memory marker CD45RO on helper T cells in macaques.
Dual role of miR-21 in CD4+ T-cells: activation-induced miR-21 supports survival of memory T-cells and regulates CCR7 expression in naive T-cells.
Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function.
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.
Impaired CD4 and CD8 effector function and decreased memory T cell populations in ICOS-deficient patients.
Emami-Shahri N, Foster J, Kashani R, Gazinska P, Cook C, Sosabowski J, Maher J, Papa S
Nature communications 2018 Mar 14;9(1):1081
Nature communications 2018 Mar 14;9(1):1081
Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Somatic Variation of T-Cell Receptor Genes Strongly Associate with HLA Class Restriction.
Klarenbeek PL, Doorenspleet ME, Esveldt RE, van Schaik BD, Lardy N, van Kampen AH, Tak PP, Plenge RM, Baas F, de Bakker PI, de Vries N
PloS one 2015;10(10):e0140815
PloS one 2015;10(10):e0140815
Functional expression of CD137 (4-1BB) on T helper follicular cells.
Alfaro C, Echeveste JI, Rodriguez-Ruiz ME, Solorzano JL, Perez-Gracia JL, Idoate MA, Lopez-Picazo JM, Sanchez-Paulete AR, Labiano S, Rouzaut A, Oñate C, Aznar A, Lozano MD, Melero I
Oncoimmunology 2015 Dec;4(12):e1054597
Oncoimmunology 2015 Dec;4(12):e1054597
Delta-like 1-mediated Notch signaling enhances the in vitro conversion of human memory CD4 T cells into FOXP3-expressing regulatory T cells.
Mota C, Nunes-Silva V, Pires AR, Matoso P, Victorino RM, Sousa AE, Caramalho I
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 15;193(12):5854-62
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 15;193(12):5854-62
Expression of the memory marker CD45RO on helper T cells in macaques.
Valentine M, Song K, Maresh GA, Mack H, Huaman MC, Polacino P, Ho O, Cristillo A, Kyung Chung H, Hu SL, Pincus SH
PloS one 2013;8(9):e73969
PloS one 2013;8(9):e73969
Dual role of miR-21 in CD4+ T-cells: activation-induced miR-21 supports survival of memory T-cells and regulates CCR7 expression in naive T-cells.
Smigielska-Czepiel K, van den Berg A, Jellema P, Slezak-Prochazka I, Maat H, van den Bos H, van der Lei RJ, Kluiver J, Brouwer E, Boots AM, Kroesen BJ
PloS one 2013;8(10):e76217
PloS one 2013;8(10):e76217
Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function.
Ives ML, Ma CS, Palendira U, Chan A, Bustamante J, Boisson-Dupuis S, Arkwright PD, Engelhard D, Averbuch D, Magdorf K, Roesler J, Peake J, Wong M, Adelstein S, Choo S, Smart JM, French MA, Fulcher DA, Cook MC, Picard C, Durandy A, Tsumura M, Kobayashi M, Uzel G, Casanova JL, Tangye SG, Deenick EK
The Journal of allergy and clinical immunology 2013 Aug;132(2):400-11.e9
The Journal of allergy and clinical immunology 2013 Aug;132(2):400-11.e9
CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.
Landrigan A, Wong MT, Utz PJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):3033-43
Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.
Teirlinck AC, McCall MB, Roestenberg M, Scholzen A, Woestenenk R, de Mast Q, van der Ven AJ, Hermsen CC, Luty AJ, Sauerwein RW
PLoS pathogens 2011 Dec;7(12):e1002389
PLoS pathogens 2011 Dec;7(12):e1002389
Impaired CD4 and CD8 effector function and decreased memory T cell populations in ICOS-deficient patients.
Takahashi N, Matsumoto K, Saito H, Nanki T, Miyasaka N, Kobata T, Azuma M, Lee SK, Mizutani S, Morio T
Journal of immunology (Baltimore, Md. : 1950) 2009 May 1;182(9):5515-27
Journal of immunology (Baltimore, Md. : 1950) 2009 May 1;182(9):5515-27
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Anti-Human CD45RO PE. Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
