Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-9979-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD45RA Monoclonal Antibody (JS-83), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The JS-83 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4^+ peripheral T lymphocytes, CD8^+ peripheral T lymphocytes, and B cells. The CD45RA^+ T cell populations are mainly naive/virgin allowing the use of JS-83 mAb as a phenotypic marker to discriminate T cell subsets. Applications Reported: JS-83 has been reported for use in flow cytometric analysis. Applications Tested: This JS-83 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- JS-83
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Human immunodeficiency virus type I-specific CD8+ T cell subset abnormalities in chronic infection persist through effective antiretroviral therapy.
TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.
Pohling J, Zipperlen K, Hollett NA, Gallant ME, Grant MD
BMC infectious diseases 2010 May 25;10:129
BMC infectious diseases 2010 May 25;10:129
TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.
Arimura Y, Vang T, Tautz L, Williams S, Mustelin T
Molecular immunology 2008 Jun;45(11):3074-84
Molecular immunology 2008 Jun;45(11):3074-84
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Surface staining of normal human peripheral blood cells with Anti-Human CD45RA FITC. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
- Conjugate
- Yellow dye