Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 86-0452-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD45R (B220) Monoclonal Antibody (RA3-6B2), eVolve 655, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RA3-6B2 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- RA3-6B2
- Vial size
- 25 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A role for brain-derived neurotrophic factor in B cell development.
Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
Schuhmann B, Dietrich A, Sel S, Hahn C, Klingenspor M, Lommatzsch M, Gudermann T, Braun A, Renz H, Nockher WA
Journal of neuroimmunology 2005 Jun;163(1-2):15-23
Journal of neuroimmunology 2005 Jun;163(1-2):15-23
Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
Monteith CE, Chelack BJ, Davis WC, Haines DM
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1996 Jul;60(3):193-8
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1996 Jul;60(3):193-8
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of mouse splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and Anti-Human/Mouse CD45R (B220) eVolve™ 655. Total cells were used for analysis.