86-0452-41

antibody from Invitrogen Antibodies
Targeting: PTPRC CD45, GP180, LCA, T200
Provider product page for 86-0452-41

Antibody data

Product number
86-0452-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD45R (B220) Monoclonal Antibody (RA3-6B2), eVolve 655, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RA3-6B2 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve™ 655 can be optimally excited with laser lines from 325-405 nm, and emits near 655 nm. We recommend using the violet (405 nm) laser line for excitation and a 630LP with a 660/40 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-635 nm; Emission: 657 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
RA3-6B2
Vial size
25 Tests
Concentration
5 µl/Test
Storage
4° C, store in dark, DO NOT FREEZE!
PTPRC protein structure - 86-0452-41 shown in red.