Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-32956-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Chromogranin A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Porcine, Rabbit, Sheep, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (LK2H10)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Mouse Pancreas stained with Chromogranin A Antibody (LK2H10).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Chromogranin A Antibody (LK2H10) [NBP2-32956] - Flow Cytometry: Chromogranin A Antibody (LK2H10) [Alexa Fluor® 647] [NBP2-34672AF647] - An intracellular stain was performed on SH-SY5Y cells with Chromogranin A Antibody (LK2H10) NBP2-34672AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.