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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-35310 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ErbB2 (HER-2) (Tyr1005) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ERBB2 in extracts from A431 cells, untreated or treated with EGF, using a Phospho-ERBB2 Tyr1005 polyclonal antibody (Product # PA5-35310) (left) and an unphosphorylated ERBB2 antibody (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-ERBB2 Tyr1005 showing staining in the membrane and cytoplasm of MCF-7 cells. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and probed with a Phospho-ERBB2 Tyr1005 polyclonal antibody (Product # PA5-35310) at a dilution of 1:100 for 2 hours at room temperature, followed by detection using a fluorescent conjugated secondary antibody (green) (1:1000, 1h). Nuclei were stained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
