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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AM33074PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rat Monoclonal Anti-MKI67 Antibody, Purified
- Antibody type
- Monoclonal
- Description
- Rat Monoclonal Anti-MKI67 Antibody, Purified
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Clone 16A8
- Vial size
- 25ug
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis: Whole cell extracts of P3X63Ag8 Mouse myeloma cells was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti Ki-67 Antibody -N (Clone 16A8). Proteins were visualized using an anti-Rat-IgG secondary conjugated to HRP and chemiluminescence detection.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent microscope analysis of P3X63Ag8 mouse myeloma cells using anti Ki-67 Antibody (Clone 16A8) antibody (green). Nuclei were stained with DAPI (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FFPE Mouse spleen was stained with Purified Anti-Mouse Ki-67 Antibody -N or Purified Rat IgG2a, ? at 1/50 (10 ug/mL) using 10 mM Sodium Citrate Buffer pH 6.0 for antigen retrieval and polymer detection system. Cellular localization: Nuclear. Left: Positive Ctrl, FFPE Mouse Spleen, 20X Magnification.? Right: Negative Ctrl, FFPE Mouse Spleen, 20X Magnification.
- Validation comment
- IHC
