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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC7617G-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Human Ki67/MKI67 Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human Ki-67/MKI67 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Green dye
- Antigen sequence
P46013- Isotype
- IgG
- Antibody clone number
- 1297A
- Vial size
- 100 ug
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Ki-67/MKI67 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 μg/mL PHA for 5 days, or (B) untreated, were stained with Rabbit Anti-Human Ki-67/MKI67 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC7617G) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC1051G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
