Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-15525 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ki-67 Monoclonal Antibody (4A1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-15525 targets KI67 in IHC applications and shows reactivity with Human samples.
- Antibody clone number
- 4A1
- Concentration
- Conc. Not Determined
Submitted references Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma.
Mammary serine protease inhibitor and CD138 immunohistochemical expression in ovarian serous and clear cell carcinomas.
Notch3 pathway alterations in ovarian cancer.
Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer.
Wang L, Yang C, Wang Q, Liu Q, Wang Y, Zhou J, Li Y, Tan Y, Kang C
Theranostics 2020;10(15):6674-6694
Theranostics 2020;10(15):6674-6694
Mammary serine protease inhibitor and CD138 immunohistochemical expression in ovarian serous and clear cell carcinomas.
Hasby EA
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Apr;37(4):4889-900
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Apr;37(4):4889-900
Notch3 pathway alterations in ovarian cancer.
Hu W, Liu T, Ivan C, Sun Y, Huang J, Mangala LS, Miyake T, Dalton HJ, Pradeep S, Rupaimoole R, Previs RA, Han HD, Bottsford-Miller J, Zand B, Kang Y, Pecot CV, Nick AM, Wu SY, Lee JS, Sehgal V, Ram P, Liu J, Tucker SL, Lopez-Berestein G, Baggerly KA, Coleman RL, Sood AK
Cancer research 2014 Jun 15;74(12):3282-93
Cancer research 2014 Jun 15;74(12):3282-93
Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer.
Qiu ZX, Wang L, Han J, Liu D, Huang W, Altaf K, Qiu XS, Javed MA, Zheng J, Chen BJ, Li WM
Cancer science 2012 Oct;103(10):1774-9
Cancer science 2012 Oct;103(10):1774-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Ki67 was performed using 70% confluent log phase HeLa cells serum starved for 36 Hrs followed by serum release for 6 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Ki-67 Monoclonal Antibody (4A1) (Product # MA5-15525) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents serum starved cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D) using KI67 monoclonal antibody (Product # MA5-15525) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 eGFP-Cavin1 was transferred via EVs to recipient LN229 cells and increased LN229 proliferation in orthotopic xenograft glioma mice. (A) Schematic illustration of experimental grouping and process of the mixed glioma xenograft model. U87-eGFP, U87-C, and U87-vC were respectively mixed with an equal number of LN229-RFP-luc and implanted intracranially in nude mice. IVIS detection was performed at day 7, 14, 21, and 28 post-implantation (n = 8), and brains were dissociated at day 21 for histological analysis and confocal imaging. (B) In vivo bioluminescence imaging showing a higher signal intensity in mice implanted with LN229-RFP-luc+U87-C. (C) Analysis of the bioluminescence intensity suggesting a rapidly increasing growth of LN229-RFP-luc which were mixed with U87-C from day 7. (D) Weight analysis indicating a faster weight loss in mice implanted with LN229-RFP-luc+U87-C from day 12 (n = 8). (E) Kaplan-Meier survival curves showing the percent survival of mice implanted with LN229-RFP-luc+U87-eGFP, LN229-RFP-luc+U87-C, and LN229-RFP-luc+U87-vC, respectively (n = 8, p < 0.001; log-rank test). (F) H&E and Ki67 staining of mouse cerebrum with tumor which was harvested at day 21 post implantation (n = 5). H&E staining showing a more heterogeneous composition in the LN229-RFP-luc+U87-C tumor. Scale bar, 100 um. (G) IHC for Ki67 showing an increased number of Ki67-positive cells in the LN229-RFP-luc+U87-C tumor (mean+-SEM, p < 0.0001; p < 0.0001). (H) 3D images generated f