GTX83641

antibody from GeneTex

Targeting: PMEL D12S53E, gp100, Pmel17, SI, SIL, SILV

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX83641

Provider

GeneTex

Proper citation

GeneTex Cat#GTX83641, RRID:AB_10728192

Product name

Melanoma gp100 antibody [9G3]

Antibody type

Monoclonal

Reactivity

Human, Canine

Host

Mouse

Storage

For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SILV (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SILV .

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SILV monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-SILV mouse monoclonal antibody (GTX83641) immunofluorescent staining of COS7 cells transiently transfected with SILV