PA5-36020

antibody from Invitrogen Antibodies

Targeting: CASP9 APAF-3, ICE-LAP6, MCH6, PPP1R56

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36020

Provider

Invitrogen Antibodies

Product name

Phospho-Caspase 9 (Tyr153) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 35 and 48 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Caspase 9 (Tyr153) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with UV for 1h whole cell lysate (40 µg), Lane 3: HEK293T treated with UV for 6h whole cell lysate (40 µg), Lane 4: HEK293T treated with UV for 16h whole cell lysate (40 µg), Lane 5: HEK293T treated with UV for 24h whole cell lysate (40 µg). Samples were incubated with Phospho-Caspase 9 (Tyr153) polyclonal antibody (Product # PA5-36020) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Caspase 9 (Tyr153) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with UV for 1h whole cell lysate (40 µg), Lane 3: HEK293T treated with UV for 6h whole cell lysate (40 µg), Lane 4: HEK293T treated with UV for 16h whole cell lysate (40 µg), Lane 5: HEK293T treated with UV for 24h whole cell lysate (40 µg). Samples were incubated with Phospho-Caspase 9 (Tyr153) polyclonal antibody (Product # PA5-36020) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Caspase 9 pTyr153 using Phospho-Caspase 9 pTyr153 polyclonal antibody (Product # PA5-36020) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: PC12 whole cell lysate.