Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-15531 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal B-Raf Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody [NBP2-15531] - Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody [NBP2-15531] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B Raf antibody diluted by 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody [NBP2-15531] - A. 30 ug PC-12 whole cell extract 7.5 % SDS-PAGE B-Raf antibody [N2C1], Internal dilution: 1:5000
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: B-Raf Antibody [NBP2-15531] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 ug of B-Raf antibody [N2C1], Internal 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-15531] - Paraffin-embedded mouse testis. B-Raf antibody [N2C1], Internal dilution: 1:500.