AP3418a

antibody from Abgent

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3418a

Provider

Abgent

Proper citation

Abgent Cat#AP3418a, RRID:AB_1277960

Product name

Phospho-BRAF(S445) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.4 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Dot blot analysis of anti-BRAF-pS445 Phospho-specific Pab (Cat.#AP3418a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Primary Ab dilution

1:500