Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-41219 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal B-Raf Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf a has one isoform (712aa, 80kD). NBP2-41219 can detect in human B-raf and mouse isoform 1. This antibody will not cross-react with C-raf.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody [NBP2-41219] - Analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: B-Raf Antibody [NBP2-41219] - 15 ug of lysates per lane. Antibodies: B-raf 5777, (0.5 ug/mL), B-raf 7379, (0.5 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antichicken and goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: B-Raf Antibody [NBP2-41219] - Human small intestine tissue with B-raf antibody at 2.5 ug/ml.
