LS-C368803

antibody from LSBio

Targeting: BRAF BRAF1

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C368803

Provider

LSBio

Product name

BRAF / B-Raf Antibody (phospho-Ser446) LS-C368803

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemical analysis of B-RAF (pS446) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical analysis of B-RAF (pS446) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemical analysis of B-RAF (pS446) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.