M00075

antibody from Boster Biological Technology

Targeting: BRAF BRAF1

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00075

Provider

Boster Biological Technology

Product name

Anti-B Raf Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for RAF B/BRAF detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IHC, WB. Reactive species: Human, Mouse, Rat RAF B/BRAF information: Molecular Weight: 84437 MW; Subcellular Localization: Nucleus . Cytoplasm. Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes; Tissue Specificity: Brain and testis.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

CCG-2

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody(M00075)BRAF was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BRAF Antibody (M00075)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image