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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- R20306 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant BRAF V600E Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified from animal origin-free supernatant
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM8
- Vial size
- 25 µg
- Concentration
- 1 mg/ml
- Storage
- Store the recombinant BRAF V600E antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein with recombinant BRAF V600E antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC staining of formalin fixed and paraffin embedded human WiDr cells (400X, Positive) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC staining of formalin fixed and paraffin embedded human SW40 cells (400X, Negative) with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of formalin fixed and paraffin embedded melanoma tissue sections with recombinant BRAF V600E antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be positive for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of formalin fixed and paraffin embedded thyroid carcinoma tissue with recombinant BRAF V600E antibody. Allele specific PCR validated to be negative for BRAF V600E. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
