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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-31548 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OGFOD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: mouse brain, rat brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OGFOD1 using 30 µg of IMR32 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an OGFOD1 polyclonal antibody (Product # PA5-31548) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OGFOD1 was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a OGFOD1 Polyclonal Antibody (Product # PA5-31548) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of OGFOD1 was performed in paraffin-embedded mouse brain tissue using OGFOD1 Polyclonal Antibody (Product # PA5-31548) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of OGFOD1 was performed in frozen sectioned E13.5 Rat brain tissue using OGFOD1 Polyclonal Antibody (Product # PA5-31548) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.
