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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-49956 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-49956, RRID:AB_10012146
- Product name
- Rabbit Polyclonal p62/SQSTM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The signaling pathways implicated in impairment of hepatic autophagy in glycogen storage disease type Ia.
Activation of tumor-promoting pathways implicated in hepatocellular adenoma/carcinoma, a long-term complication of glycogen storage disease type Ia.
NSAIDs disrupt intestinal homeostasis by suppressing macroautophagy in intestinal epithelial cells.
Gene therapy prevents hepatic tumor initiation in murine glycogen storage disease type Ia at the tumor-developing stage.
Lysosomal trapping of palbociclib and its functional implications.
p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors.
Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation.
Metastatic risk and resistance to BRAF inhibitors in melanoma defined by selective allelic loss of ATG5.
Potentiation of neurotoxicity in double-mutant mice with Pink1 ablation and A53T-SNCA overexpression.
Blocking IGF Signaling in Adult Neurons Alleviates Alzheimer's Disease Pathology through Amyloid-β Clearance.
Gautam S, Zhang L, Arnaoutova I, Lee C, Mansfield BC, Chou JY
Human molecular genetics 2020 Mar 27;29(5):834-844
Human molecular genetics 2020 Mar 27;29(5):834-844
Activation of tumor-promoting pathways implicated in hepatocellular adenoma/carcinoma, a long-term complication of glycogen storage disease type Ia.
Cho JH, Lee YM, Bae SH, Chou JY
Biochemical and biophysical research communications 2020 Jan 29;522(1):1-7
Biochemical and biophysical research communications 2020 Jan 29;522(1):1-7
NSAIDs disrupt intestinal homeostasis by suppressing macroautophagy in intestinal epithelial cells.
Chamoun-Emanuelli AM, Bryan LK, Cohen ND, Tetrault TL, Szule JA, Barhoumi R, Whitfield-Cargile CM
Scientific reports 2019 Oct 10;9(1):14534
Scientific reports 2019 Oct 10;9(1):14534
Gene therapy prevents hepatic tumor initiation in murine glycogen storage disease type Ia at the tumor-developing stage.
Cho JH, Lee YM, Starost MF, Mansfield BC, Chou JY
Journal of inherited metabolic disease 2019 May;42(3):459-469
Journal of inherited metabolic disease 2019 May;42(3):459-469
Lysosomal trapping of palbociclib and its functional implications.
Llanos S, Megias D, Blanco-Aparicio C, Hernández-Encinas E, Rovira M, Pietrocola F, Serrano M
Oncogene 2019 May;38(20):3886-3902
Oncogene 2019 May;38(20):3886-3902
p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors.
Karras P, Riveiro-Falkenbach E, Cañón E, Tejedo C, Calvo TG, Martínez-Herranz R, Alonso-Curbelo D, Cifdaloz M, Perez-Guijarro E, Gómez-López G, Ximenez-Embun P, Muñoz J, Megias D, Olmeda D, Moscat J, Ortiz-Romero PL, Rodríguez-Peralto JL, Soengas MS
Cancer cell 2019 Jan 14;35(1):46-63.e10
Cancer cell 2019 Jan 14;35(1):46-63.e10
Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation.
Goiran T, Duplan E, Rouland L, El Manaa W, Lauritzen I, Dunys J, You H, Checler F, Alves da Costa C
Cell death and differentiation 2018 May;25(5):873-884
Cell death and differentiation 2018 May;25(5):873-884
Metastatic risk and resistance to BRAF inhibitors in melanoma defined by selective allelic loss of ATG5.
García-Fernández M, Karras P, Checinska A, Cañón E, Calvo GT, Gómez-López G, Cifdaloz M, Colmenar A, Espinosa-Hevia L, Olmeda D, Soengas MS
Autophagy 2016 Oct 2;12(10):1776-1790
Autophagy 2016 Oct 2;12(10):1776-1790
Potentiation of neurotoxicity in double-mutant mice with Pink1 ablation and A53T-SNCA overexpression.
Gispert S, Brehm N, Weil J, Seidel K, Rüb U, Kern B, Walter M, Roeper J, Auburger G
Human molecular genetics 2015 Feb 15;24(4):1061-76
Human molecular genetics 2015 Feb 15;24(4):1061-76
Blocking IGF Signaling in Adult Neurons Alleviates Alzheimer's Disease Pathology through Amyloid-β Clearance.
Gontier G, George C, Chaker Z, Holzenberger M, Aïd S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Aug 19;35(33):11500-13
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Aug 19;35(33):11500-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody [NBP1-49956] - Detection of Human and Mouse Sequestosome-1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Sequestosome-1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Sequestosome-1 was also immunoprecipitated by rabbit anti-Sequestosome-1 antibodies which recognize upstream epitopes. For blotting immunoprecipitated Sequestosome-1 was used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody [NBP1-49956] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Sequestosome-1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate
