Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-42822 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-42822, RRID:AB_2196010
- Product name
- Rabbit Polyclonal p62/SQSTM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Polycystin-2 Is Required for Starvation- and Rapamycin-Induced Atrophy in Myotubes.
Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells.
Hyperosmotic stress stimulates autophagy via polycystin-2.
Neuroprotective Effects of β-Asarone Against 6-Hydroxy Dopamine-Induced Parkinsonism via JNK/Bcl-2/Beclin-1 Pathway.
Kretschmar C, Peña-Oyarzun D, Hernando C, Hernández-Moya N, Molina-Berríos A, Hernández-Cáceres MP, Lavandero S, Budini M, Morselli E, Parra V, Troncoso R, Criollo A
Frontiers in endocrinology 2019;10:280
Frontiers in endocrinology 2019;10:280
Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells.
Lin YJ, Liang WM, Chen CJ, Tsang H, Chiou JS, Liu X, Cheng CF, Lin TH, Liao CC, Huang SM, Chen J, Tsai FJ, Li TM
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 May;58:152893
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 May;58:152893
Hyperosmotic stress stimulates autophagy via polycystin-2.
Peña-Oyarzun D, Troncoso R, Kretschmar C, Hernando C, Budini M, Morselli E, Lavandero S, Criollo A
Oncotarget 2017 Aug 22;8(34):55984-55997
Oncotarget 2017 Aug 22;8(34):55984-55997
Neuroprotective Effects of β-Asarone Against 6-Hydroxy Dopamine-Induced Parkinsonism via JNK/Bcl-2/Beclin-1 Pathway.
Zhang S, Gui XH, Huang LP, Deng MZ, Fang RM, Ke XH, He YP, Li L, Fang YQ
Molecular neurobiology 2016 Jan;53(1):83-94
Molecular neurobiology 2016 Jan;53(1):83-94
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: p62/SQSTM1 Antibody [NBP1-42822] - Simple Western lane view shows a specific band for p62/SQSTM1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody [NBP1-42822] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml anti-p62/SQSMT1 in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.
