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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 14-6508-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Chorionic Gonadotropin Monoclonal Antibody (FB12), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This FB12 monoclonal antibody reacts with human Chorionic Gonadotropin (hCG). This secreted glycoprotein is composed of an alpha subunit, which is common to other peptide hormones such as luteinizing hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH), and a beta subunit which determines the specificity of the hormone. FB12 recognizes both the intact hCG (composed of the alpha and beta subunits) as well as the free beta subunit, highly expressed in both choriocarcinoma and the serum of pregnant women. hCG is normally expressed by trophoblastoid cells (syncytiotrophoblasts and extravillous cytotrophoblasts) that are responsible for maintenance of the corpus luteum during early gestation. hCG plays an important role in the regulation of angiogenesis through activation of the PI3K/mTOR pathway, resulting in upregulation of vascular endothelial growth factor (VEGF). Ectoptic expression of hCG has also been reported in multiple types of cancer (bladder, lung, breast, pancreas, renal).
- Antibody clone number
- FB12
- Concentration
- 0.5 mg/mL
Submitted references Mechanobiological regulation of placental trophoblast fusion and function through extracellular matrix rigidity.
Ma Z, Sagrillo-Fagundes L, Mok S, Vaillancourt C, Moraes C
Scientific reports 2020 Apr 3;10(1):5837
Scientific reports 2020 Apr 3;10(1):5837
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry on fixed BeWo cells using 5 µg/mL Mouse IgG1 K Isotype Control (left) or 5 µg/mL Anti-Human Chorionic Gonadotropin Purified (right) followed by Anti-Mouse TRITC.Nuclei are counterstained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effects of substrate stiffness on BeWo cell beta-hCG expression. ( a , b ) BeWo cells only express beta-hCG with forskolin treatment. ( c , d ) Representative figures of cells expressing beta-hCG on soft ( c ) and stiff ( d ) substrates. Red: beta-hCG; blue: DAPI. Scale bar is 50 mum. ( e ) Ratio of BeWo cells expressing beta-hCG and ( f ) normalized media beta-hCG level when cultured on substrates with various stiffness. Data reported as mean +- standard deviation for n = 3 independent experiments; *p < 0.05, **p < 0.01, ***p < 0.001, by two-tailed, one-way ANOVA with Holm-Sidak Post-hoc comparison.
