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LearnLS-B10262
antibody from LSBio
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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- Product number
- LS-B10262 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 11G2) LS-B10262
- Antibody type
- Monoclonal
- Description
- Protein A/G purified from tissue culture supernatant.
- Reactivity
- Human, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 11G2
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-SERPINA1 / Alpha 1 Antitrypsin antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
