TA319139
antibody from OriGene
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
Reference
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- Product number
- TA319139 - Provider product page
- Provider
- OriGene
- Product name
- Goat polyclonal Alpha 1 Anti Trypsin antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal Alpha 1 Anti Trypsin antibody
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- SERPINA1
- Antibody clone number
- NULL
- Vial size
- 2 ml
- Concentration
- 90 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Goat anti Alpha-1anti-Trypsin antibody was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat 1:10K in TBS/MB-070 1 hr RT).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Goat-anti-Alpha-1-Anti-Trypsin (TA319139, red), Rabbit anti-Transferrin , and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Membrane was probed with three primary antibodies at 1:1000 dilution. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG , and DyLight 649 Donkey anti-Goat IgG.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin ; Alpha 1 anti trypsin and Human IgG in a multiplex fluorescent WB of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~ 1hr at 4 degrees.
- Validation comment
- WB