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LearnNBP1-78098-0.1mg
antibody from Novus Biologicals
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- NBP1-78098-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Serpin A1/alpha 1-Antitrypsin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-78098] - Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in buffer over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (shown as green) DyLight488 Donkey anti-Mouse IgG (shown as blue), and DyLight649 Donkey anti-Goat IgG (shown as red) at 1:10000 (in buffer 30 min RT).
