PA5-51544

antibody from Invitrogen Antibodies

Targeting: CBS HIP4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51544

Provider

Invitrogen Antibodies

Product name

CBS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GGSVKDRISL RMIEDAERDG TLKPGDTIIE PTSGNTGIGL ALAAAVRGYR CIIVMPEKMS SEKVDVLRAL GAEIVRTPTN ARFDSPESHV GVAWRLKNEI PNSHILDQYR NASNPLAHYD TTADEILQQC DGKLDMLVAS VGTGGTITGI GAPHR

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CBS Polyclonal Antibody(Product # PA5-51544) and a 55kDa band corresponding to CBS was observed across cell lines and tissues tested except in Rat Heart which is reported to be negative. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), HEK-293 (Lane 2) and tissue extracts (30 ug lysate) of Rat Liver (Lane 3) and Rat Heart (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band of ~80 kDa was seen in the tested cell lines.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of CBS in human cell line U-251 MG shows positivity in nucleoli & vesicles. Samples were probed using a CBS Polyclonal Antibody (Product # PA5-51544).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CBS in human liver using a CBS Polyclonal Antibody (Product # PA5-51544) shows moderate cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CBS in human pancreas using a CBS Polyclonal Antibody (Product # PA5-51544) shows moderate cytoplasmic positivity in exocrine glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CBS in human cerebellum using a CBS Polyclonal Antibody (Product # PA5-51544) shows moderate cytoplasmic positivity in a subset of cells in granular layer, as well as in neuropil of molecular layer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CBS in human skeletal muscle using a CBS Polyclonal Antibody (Product # PA5-51544) shows no positivity in striated muscle fibers as expected.