Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-76031 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CBS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CBS in extracts of mouse kidney tissue. Samples were incubated with CBS polyclonal antibody (Product # PA5-76031).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CBS Polyclonal Antibody (Product # PA5-76031) and a band at 61 kDa corresponding to CBS was observed across the cell lines and tissue tested. OVCAR-3 was observed to be high expressing cell line when compared to SK-OV-3; Mouse, Rat Liver and Mouse Pancreas were observed to be high expressing tissues when compared to Mouse and Rat Heart as reported in the literature. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), HeLa (Lane 3), OVCAR-3 (Lane 4), SK-OV-3 (Lane 5), DU 145 (Lane 6), tissue extracts of Mouse Liver (Lane 7), Rat Liver (Lane 8), Mouse Pancreas (Lane 9), Mouse Heart (Lane 10) and Rat Heart (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CBS in U2OS cells. Samples were incubated with CBS polyclonal antibody (Product # PA5-76031).