AB-201-NA

antibody from Novus Biologicals

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-201-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-1 beta/IL-1F2 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects human IL-1 beta/IL-1F2 in ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant rhesus monkey is observed, and approximately 50% cross-reactivity with recombinant mouse IL-1 beta , recombinant rat IL-1 beta , recombinant canine IL-1 beta , recombinant equine IL-1 beta , recombinant feline IL-1 beta , and approximately 20% cross-reactivity with recombinant porcine IL-1 beta and recombinant cotton rat IL-1 beta is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.

Solanilla A, Déchanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
Blood 2000 Jun 15;95(12):3758-64

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse IL-1 beta/IL-1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta/IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Human IL-1 beta/ IL-1F2 Antibody. Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta/ IL-1F2 (50 pg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta/IL-1F2 Poly-clonal Antibody (Catalog # AB-201-NA). The ND₅₀ is typically 0.05-0.1 µg/mL in the presence of concanavalin A (1.25 µg/mL).