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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF3747 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine IL-1 beta in direct ELISAs and Western blots.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
Cytokine 2008 Dec;44(3):352-60
Cytokine 2008 Dec;44(3):352-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Canine IL-1 beta/IL-1F2 Antibody. Recombinant Canine IL-1 beta/IL-1F2 (Catalog # 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-1 beta/IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3747). The ND50 is typically 2-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).
