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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAF501 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IL-1 beta/IL-1F2 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat IL-1 beta/IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.8% cross-reactivity with recombinant mouse (rm) IL-1 beta is observed and less than 0.2% cross-reactivity with recombinant human (rh) IL-1 alpha , recombinant rat IL-1 alpha , recombinant porcine (rp) IL-1 alpha , rhIL-1 beta , rpIL-1 beta , rhIL-1 RA, rmIL-1 RA, rpIL-1 RA, rhIL-1 RII, rmIL-1 RI Fc Chimera, rhIL-1 Rrp2 Fc Chimera, and rhIL-1 RAcP Fc Chimera is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q63264- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues.
Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
García-Pupo L, Zaldo-Castro A, Exarchou V, Tacoronte-Morales JE, Pieters L, Vanden Berghe W, Nuñez-Figueredo Y, Delgado-Hernández R
Molecules (Basel, Switzerland) 2016 Jul 29;21(8)
Molecules (Basel, Switzerland) 2016 Jul 29;21(8)
Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
Infection and immunity 2008 Aug;76(8):3577-86
Infection and immunity 2008 Aug;76(8):3577-86
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Roche M, Diamond M, Kelly JP, Finn DP
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-1 beta/IL-1F2 in Rat Splenocytes. IL-1 beta/IL-1F2 was detected in immersion fixed rat splenocytes treated with PMA and calcium ionomycin using Goat Anti-Rat IL-1 beta/IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF501) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
