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Immunocytochemistry
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB501 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human IL-1 alpha , recombinant mouse IL-1 alpha , and recombinant rat IL-1 alpha is observed.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q63264- Isotype
- IgG
- Antibody clone number
- 38139
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A.
Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation.
Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation.
Joyal JS, Sitaras N, Binet F, Rivera JC, Stahl A, Zaniolo K, Shao Z, Polosa A, Zhu T, Hamel D, Djavari M, Kunik D, Honoré JC, Picard E, Zabeida A, Varma DR, Hickson G, Mancini J, Klagsbrun M, Costantino S, Beauséjour C, Lachapelle P, Smith LE, Chemtob S, Sapieha P
Blood 2011 Jun 2;117(22):6024-35
Blood 2011 Jun 2;117(22):6024-35
Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation.
Morelli AE, Zahorchak AF, Larregina AT, Colvin BL, Logar AJ, Takayama T, Falo LD, Thomson AW
Blood 2001 Sep 1;98(5):1512-23
Blood 2001 Sep 1;98(5):1512-23
Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation.
Morelli AE, Zahorchak AF, Larregina AT, Colvin BL, Logar AJ, Takayama T, Falo LD, Thomson AW
Blood 2001 Sep 1;98(5):1512-23
Blood 2001 Sep 1;98(5):1512-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Rat IL-1 beta/IL-1F2 Antibody. Recombinant Rat IL-1 beta/IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta/IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND50 is typically 10-30 µg/mL in the presence of concanavalin A (1.25 µg/mL).
