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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF3340 - Provider product page
- Provider
- R&D Systems
- Product name
- Equine IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects equine IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant canine IL-1 beta , recombinant feline IL-1 beta , and recombinant human IL-1 beta is observed, 40% cross-reactivity with recombinant porcine IL-1 beta , recombinant mouse IL-1 beta , and recombinant rat IL-1 beta is observed, and 10% cross-reactivity with recombinant cotton rat IL-1 beta and is observed.
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q28386- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 beta/IL-1F2 in Equine PBMCs. IL-1 beta/IL-1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3340) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Equine IL-1 beta/IL-1F2 Antibody. Recombinant Equine IL-1 beta/ IL-1F2 (Catalog # 3340-EL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-1 beta/IL-1F2 (100 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-1 beta/IL-1F2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3340). The ND50 is typically 0.05-0.25 µg/mL in the presence of concanavalin A (1.25 µg/mL).
