MA1-779

antibody from Invitrogen Antibodies

Targeting: MMP2 CLG4, CLG4A, TBE-1

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-779

Provider

Invitrogen Antibodies

Product name

MMP2 Monoclonal Antibody (MMP2/2C1-1D12)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-779 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

Antibody clone number

MMP2/2C1-1D12

Concentration

1 mg/mL

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MMP2 showing staining in the cytoplasm and membrane of paraffin-embedded human breast tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a MMP2 monoclonal antibody (Product # MA1-779) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.